The set up for fluid imaging is very similar to set up for other AFM operations, with a few key differences. Fluid imaging is not possible without proper set up of the appropriate hardware.
Ensure that you have chosen the "Fluid" option for the imaging mode of your choice in the Experiment Group panel.
A special probe holder must be used for imaging in fluid with the Dimension Icon scanner. See Fluid Operation Hardware for more information.
Ensure that you reset the parameters to their default values (12) when using the standard air tapping or TR holder.
The cantilever probe position extends roughly 1 mm further toward the sample with the fluid probe holder, as compared with the standard cantilever holder.
If the height of the scanner needs adjustment, use one of the following methods:
The protective skirt is a rubber seal used to protect the SPM scanner tube from liquids. Install it by sliding it over the fluid probe holder and onto the shoulder on the SPM tube. Ensure that the seal is tight on both the probe holder and the SPM tube. Below is an illustration of the SPM head and the fluid probe holder with the protective skirt installed:
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